Institutional facilities at Greenarray

Greenarray has state of the art laboratory with high quality standards. The laboratory has advanced set up for molecular testing and diagnostic requirements. The lab has cutting-edge sequencing technologies like PCR equipment, Sanger sequencer, Microarray.

DNA & RNA EXTRACTION

We offer DNA and RNA extraction services from different samples like environmental and clinical and their storage at -20 an -80°C.

16S rRNA gene sequencing

Ribosomal RNA (rRNA) gene sequence of bacteria is one of the most reliable molecular methods of microbial identification. Greenarray offers this sequencing service for identifying bacteria with the help of universal 16S rRNA primers.

18S rRNA gene sequencing

18S rRNA gene has conserved and variable regions and widely used in molecular analysis to reconstruct the evolutionary history of organisms, especially in vertebrates, as its slow evolutionary rate makes it suitable to phylogenetic studies.

Fungal ITS sequencing

The nuclear ribosomal internal transcribed spacer (ITS) region is the most commonly chosen genetic marker for the molecular identification of fungi in environmental sequencing and molecular ecology studies.

matK/rbcL gene sequencing

rbcL and matK are the core chloroplast genes of plant species. rbcL genes have are used for phylogenetic analysis within family and subclass of angiosperm. matK is a single-copy and one of the fastest evolving genes used for studies of systematic and evolutionary botany

QF-PCR

Quantitative fluorescent polymerase chain reaction (QF-PCR) provides a targeted and rapid diagnosis of chromosomal abnormalities. It has the advantages over the cytogenetic culture of being faster with the results appearing within 24–48 hours.

COI gene sequencing

For animal identification, the most broadly used barcode marker is mitochondrial cytochrome c oxidase subunit I (COI), which is highly conserved across species employing oxidative phosphorylation for metabolism.
COI-based DNA barcoding can delimit diverse animal species, indicating the high rates of sequence change at species level and constraints on intraspecific divergence in COI sequence (a 658-bp region of COI gene)

Targeted gene sequencing

Targeted gene sequencing are useful tools for analysing specific mutations in the gene of interest of given sample. Identifying heterozygous base positions or small insertions or deletions in genomic DNA is often employed to locate mutations or polymorphisms in diploid organisms. Targeted gene sequencing may contain set of genes or single gene regions that have known or suspected associations with the disease or phenotype under study. The gene of interest that can be analysed up to 1200bp region.

Fragment Analysis (Microsatellite)

Microsatellite markers are co-dominant, polymorphic DNA loci containing repeated nucleotide sequences, typically with 2 to 10 nucleotides per repeated unit. The number of nucleotides in the repeated unit is the same for the majority of the repeats within an individual microsatellite locus, but the number of repeats for a specific locus may differ, resulting in alleles of varying length, which can be analysed with fragment analysis by capillary electrophoresis.